Proteinase K Solution from Thermo Fisher Scientific

Product Specs
ItemProteinase K Solution
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog Number4333793
FormatLiquid
Brand/TrademarkInvitrogen™
Quantity5 mL
TypeProteinase K
ApplicationsChromatin Biology/RNA Extraction/DNA Extraction
PurityDNase-Free, RNase-Free

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Purification of Long Non-coding RNAs on Replication Forks Using iROND (Isolate RNAs on Nascent DNA)BIO-PROTOCOLNovember 5, 2023Weidao Zhang, Min Tang, Lin Wang, Ping Zheng, Bo Zhao)Diethyl pyrocarbonate (DEPC) (Sigma-Aldrich, catalog number: D5758)Protease K (Thermo Fisher, catalog number: 4333793)Chloroform (Sigma-Aldrich, catalog number: 366911)Isopropyl alcohol (Sigma-Aldrich, catalog number:

(2) Mode of action of antifouling compound albofungin in inhibiting barnacle larval settlementiScienceJuly 21, 2023Weiyi She, Hao Wang, Darwin Linardi, Sin Yu Chik, Yi Lan, Feng Chen, Aifang Cheng, Pei-Yuan Qian,Thermo Fisher,Cat#16070096 UltraPure™ 0.5 M EDTA (pH 8.0),Thermo Fisher,Cat#15575020 Proteinase K Solution,Thermo Fisher,Cat#4333793 Alexa fluor™ 555 phalloidin,Thermo Fisher,Cat#A34055 "Goat anti-Mouse IgG1 Cross-Adsorbed Secondary

(3) ChIP-seq profiling of H3K4me3 and H3K27me3 in an invasive insect, Bactrocera dorsalisFrontiers in GeneticsFebruary 23, 2023Yan Zhao, Juntao Hu, Jiajiao Wu, Zhihong Li0.5 M EDTA (E8030, Solarbio, China), 8 μL 1 M Tris-HCl (T1020, Solarbio, China) and 1 μL proteinase K (4333793, ThermoFisher, US) and incubating at 45°C for 2 h. Finally, DNA was purified using Bioline DNeasy minikit

(4) Deciphering Microbiota of Acute Upper Respiratory Infections: A Comparative Analysis of PCR and mNGS Methods for Lower Respiratory Trafficking PotentialAdvances in Respiratory MedicineFebruary 2, 2023Sadia Almas, Rob E. Carpenter, Anuradha Singh, Chase Rowan, Vaibhav K. Tamrakar, Rahul SharmaBriefly, samples were lysed with 340 µL of lysis buffer and 10 µL of proteinase K (Invitrogen Cat # 4333793; Thermo Fisher Scientific, Waltham, ME, USA) at 55 °C for 10 min, followed by extraction via the MP96

(5) MERS-CoV nsp1 regulates autophagic flux via mTOR signalling and dysfunctional lysosomesEmerging Microbes & InfectionsDecember 31, 2022Yujie Feng, Zhaoyi Pan, Zhihui Wang, Zhengyang Lei, Songge Yang, Huajun Zhao, Xueyao Wang, Yating Yu, Qiuju Han, Jian ZhangDNA and protein, DNase I was added and incubated for 10 min at 37 °C, and proteinase K (Invitrogen, 4333793) was added and incubated for 10 min at 55 °C. RNA was extracted using the TRIzol reagent and detected

(6) Serotonin-1A receptor, a psychiatric disease risk factor, influences offspring immunity via sex-dependent genetic nurtureiScienceDecember 22, 2022Rosa J. Chen, Anika Nabila, Swati Phalke, Danny Flores Castro, Judit Gal Toth, Paul Bergin, Jeroen Bastiaans, Heidi Stuhlmann, Alessandra B. Pernis, Miklos Tothenzymatically digested using DirectPCR Lysis Reagent (Viagen 102T) and Proteinase K (Thermo Fisher Scientific, 4333793) and DNA was extracted. Primers used for sex genotyping PCR-target the Rbm31 gene74 (Forward primer:

(7) Whole-genome sequencing of extrachromosomal circular DNA of cerebrospinal fluid of medulloblastomaFrontiers in OncologyNovember 8, 2022Yi Zhu, Zhihui Liu, Yuduo Guo, Shenglun Li, Yanming Qu, Lin Dai, Yujia Chen, Weihai Ning, Hongwei Zhang, Lixin MaL1 buffer (Plasmid Mini AX; A&A Biotechnology: #010-50), and 15 µl of Proteinase K (ThermoFisher: #4333793) was added for incubation overnight at 50°C. The lysed samples were alkaline treated and purified through

(8) Partitioned usage of chromatin remodelers by nucleosome-displacing factorsCell ReportsAugust 23, 2022Hengye Chen, Hungyo Kharerin, Archana Dhasarathy, Michael Kladde, Lu BaiCocktail,Sigma-Aldrich,Cat#P8215 Magna ChIP™ Protein A + G Magnetic Beads,Millipore Sigma,Cat#16-663 Proteinase K,Thermo Fisher,Cat#4333793 Z1005 Zymolyase,US Biological,Cat#37340-57-1 IgG Sepharose™ 6 Fast Flow,Cytiva,Cat#17-0969-01 Turbo

(9) NSD1 mediates antagonism between SWI/SNF and polycomb complexes and is required for transcriptional activation upon EZH2 inhibitionMolecular cellJuly 7, 2022Drosos Y, Myers JA, Xu B, Mathias KM, Beane EC, Radko-Juettner S, Mobley RJ, Larsen ME, Piccioni F, Ma X, et al.Dynabeads™ Protein G for Immunoprecipitation,ThermoFisher,Cat#10004D RNase A,ThermoFisher,Cat#EN0531 Ptoteinase K,ThermoFisher,Cat#4333793 Nuclease,ThermoFisher,Cat#88702 Cell titer aqueous reagent,Promega,Cat#G3582 CellTiter-Glo,Promega,Cat#G9241 Halt™ Protease and Phosphatase Inhibitor Cocktail (100X

(10) Loss of MAT2A compromises methionine metabolism and represents a vulnerability in H3K27M mutant glioma by modulating the epigenomeNature CancerApril 14, 2022Brian J. Golbourn, Matthew E. Halbert, Katharine Halligan, Srinidhi Varadharajan, Brian Krug, Nneka E. Mbah, Nisha Kabir, Ann-Catherine J. Stanton, Abigail L. Locke, Stephanie M. Casillo, et al.treated in sequence with RNase (cat. no. AM2288, Life Technologies) and then Proteinase K (cat. no. 4333793, Thermo Fisher Scientific) incubated at 65 °C for 30 min. Samples were then purified using the QIAGEN

(11) A multiplexed, next generation sequencing platform for high-throughput detection of SARS-CoV-2Nature CommunicationsDecember 1, 2021Marie-Ming Aynaud et al.buffer (1% SDS, 10 mM EDTA), 48 μL of 6 M GuHCl, and 7.25 μL proteinase K (20 mg/mL, ThermoFisher, 4333793), incubated at room temperature for 10 min and heated at 65 °C for 10 min prior to the addition of 145

(12) DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA- and DNA-Protein CrosslinksCell reportsDecember 29, 2020Saha S, Sun Y, Huang SN, Baechler SA, Pongor LS, Agama K, Jo U, Zhang H, Tse-Dinh YC, Pommier Y."Glycogen, RNA grade",ThermoFisher Scientific,Cat# R0551 "Invitrogen, Proteinase K Solution",ThermoFisher Scientific,Cat# 4333793 "Invitrogen, Proteinase K Solution (20 mg/mL), RNA grade",ThermoFisher Scientific,Cat# 25530049 N-Lauroylsarcosine sodium salt

(13) Determinants of seeding and spreading of α-synuclein pathology in the brainScience advancesNovember 1, 2020Henrich MT, Geibl FF, Lakshminarasimhan H, Stegmann A, Giasson BI, Mao X, Dawson VL, Dawson TM, Oertel WH, Surmeier DJ.in 0.1 M PB and subsequently digested at 65°C for 10 min in PBT and PK (12 μg/ml) (Invitrogen, no. 4333793). To visualize insoluble aggregates, digested sections were double stained against p-aSYN and p62 in

(14) A53T-α-synuclein overexpression in murine locus coeruleus induces Parkinson's disease-like pathology in neurons and gliaActa neuropathologica communicationsMay 10, 2018Henrich MT, Geibl FF, Lee B, Chiu WH, Koprich JB, Brotchie JM, Timmermann L, Decher N, Matschke LA, Oertel WH.0.1 M PB and subsequently digested in 0.1 M PB containing 0.3% Triton X-100 and 12 μg/ml PK (Cat. No. 4333793, Invitrogen) at 65 °C for 10 min. To visualize insoluble aggregates, digested sections were double stained

(15) Phosphoproteomic Profiling Reveals Epstein-Barr Virus Protein Kinase Integration of DNA Damage Response and Mitotic SignalingPLoS pathogensDecember 1, 2015Li R, Liao G, Nirujogi RS, Pinto SM, Shaw PG, Huang TC, Wan J, Qian J, Gowda H, Wu X, et al.adding 20 μl of Stop Buffer and incubation at 65°C for 10 min. Then 12.5 μl Proteinase K (20 mg/ml; Cat# 4333793, Invitrogen) and 25 μl 10% (w/v) SDS were added into the reaction mixtures which were incubated for

(16) Characterization of Persistent Virus-Like Particles in Two Acetate-Fed Methanogenic ReactorsPLoS ONENovember 22, 2013I-Chieh Chien, John Scott Meschke, Heidi L. Gough, John F. Fergusonwere incubated overnight in extraction buffer (100 mM EDTA, pH 8.0, 1% SDS and 1 mg/ml proteinase K (4333793, Life Technologies, Grand Island, NY)) at room temperature with gentle mixing on a rocker. The plugs

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Proteinase K Solution from Thermo Fisher Scientific

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Proteinase K Solution from Thermo Fisher Scientific

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